WesternLumaxLight  SuperiorHRP substrate

The best choice for routine, film-imaged Western blots WesternLumaxLight Superior HRP substrate is more 

sensitive than other chemiluminescent substrates, soyou can conduct shorter exposures, load less  sample, 

or use up to ten-times less antibody. Save time and money with WesternBright ECL.

Description
WesternLumaxLight Superior  is a horseradish peroxidase substrate optimized for chemiluminescent Western 
blots imaged using X-ray film. WesternLumaxLight   ECL   requires   as   little  as  ten times less antibody than 
other substrates,allowing you to save precious antibodies and samples. Additionally, the  WesternLumaxLight 
signal is long lasting,allowing multiple exposures to be carried out without substantial signal decay.

WesternLumaxLight Superior detects equal amounts of protein with up to ten times less antibody.

Duplicate slot blots containing serial dilutions of transferrin were probed with the antibody dilutions 

shown,and detected with either WesternLumaxLight Superior or Pierce ECL (Thermo Scientific) accor

-ding to the manufacturers’instructions. The blots were imaged  simultaneously  on the same piece 

of film. WesternLumaxLight Superior produced  the same signal with ten times  less primary and  five 

times less secondary antibody.

Customer feedback

After trying WesternLumaxLight Superior,we immediately switched from our usual brand and now use it on a weekly basis. 

It produces a strong, long-lasting signal with low background at a much lower cost.

Assistant Professor, Brigham Young University

WesternLumaxLight Superior is better than (the ECL substrate) I used to use because of its high sensitivity. Most  of  my 

research involves detecting low amounts of induced proteins, such as gp91 phox and HIF-1. I had some challenge to 

detect these proteins when I used other substrates;however,WesternLumaxLight Superior helps to detect these targets. 

Now, I can detect these proteins using less sample with the WesternLumaxLight Superior system. My whole lab has  now 

switched to WesternLumaxLight Superior.

Senior Research Lab Specialist, University of Michigan

We have switched to the WesternLumaxLight Superior reagent for straight western and co-IP western blotting.  We 

have used less antibodies to suit our various needs easily. By the way, the WesternBright reagent also worked beaut

-ifully during a recent demo of a competitor’s chemiluminescent imager! It performed better than the vendor‘s   own 

proprietary reagent made especially for that machine!

Lab Manager, University of North Carolina at Chapel Hill